Role of Wnt signaling pathway hypofunction mediated by dephosphorylation ofβ-catenin in impaired wound healing of type 1 diabetic rats
10.3760/cma.j.issn.1000-6699.2016.04.015
- VernacularTitle:β-catenin去磷酸化介导的Wnt信号通路功能低下在1型糖尿病大鼠创面难愈中的作用
- Author:
Xiaolei SUN
;
Yarong MA
;
Xiaoyan WANG
;
Yong LIU
;
Hong ZENG
;
Huqiang HE
;
Lei ZHANG
;
Yanzheng HE
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus;
Wound healing;
Wnt;
β-catenin
- From:
Chinese Journal of Endocrinology and Metabolism
2016;32(4):321-326
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of Wnt signaling pathway hypofunction mediated by dephosphorylation ofβ-catenin in the impaired wound healing of type 1 diabetic rats. Methods The back skin defect wounds were produced in rats with type 1 diabetes. These rats were divided into control, diabetes, lithium chloride treatment, and epidermal growth factor ( EGF) treatment groups. The situation of back wound healing, the ratio ofβ-catenin positive cells,β-catenin, phosphorylatedβ-catenin, and vascular endothelial growth factor ( VEGF) levels were detected. Results Compared to diabetes group, the wound granulation tissue was more mature, wound healing time was shorter, and healing rate, as well as the ratio ofβ-catenin positive cells, dephosphorylatedβ-catenin, and VEGF levels, were higher in normal group, lithium chloride treatment group, and EGF treatment group ( P<0. 05 or P<0. 01). Conclusion The hypofunction of Wnt signaling pathway is involved in the process of wound healing in type 1 diabetic rats, of which the dephosphorylation ofβ-catenin is the key point. EGF may play a beneficial role in the wound healing of type 1 diabetic rat models via Wnt pathway.
