Effects of liraglutide on eNOS and IRS-1 expressions in human umbilical vein endothelial cells cultured with high glucose

VernacularTitle:利拉鲁肽对高糖作用下人脐静脉内皮细胞eNOS及IRS-1表达的影响
Author: Ling YUE ; Jing DONG ; Guangda XIANG ; Linshuang ZHAO ; Junxia ZHANG ; Guangping ZHU ; Lin XIANG ; Min LIU ; Junyan LU
Publication Type:Journal Article
Keywords: Liraglutide; Human umbilical vein endothelial cells; Insulin receptor substrates-1; Endothelial nitric oxide synthase
From: Chinese Journal of Endocrinology and Metabolism 2016;32(4):327-329
CountryChina
Language:Chinese
Abstract: Humanumbilicalveinendothelialcells(HUVECs)weretreatedwith3nmol/Lliraglutidefor10, 15, 30, 45, 60, 90, 120, 150, 180, 210, 240, and 270 minutes at the concentrations of 5. 5 or 30 mmol/L glucose. Western blot analysis was used to detected protein expression and phosphorylation of insulin receptor substrates-1 ( IRS-1 ) and endothelial nitric oxide synthase ( eNOS ) . The results showed that the baseline level of phosphorylated-eNOS/eNOS was lower in high glucose group than that in normal group(0. 239 ± 0. 016 vs 0. 400 ± 0. 02,P<0. 05). Liraglutide time-dependently increased phosphorylated-eNOS/eNOS and phosphorylated-IRS-1/IRS-1 levels at 5. 5 or 30 mmol/L glucose.