In vitro expression of the pancreatic cancer specific antigen NJ001 and its clinical significance
10.3760/cma.j.issn.1009-9158.2016.04.010
- VernacularTitle:胰腺癌患者血清中 NJ001特异性抗原的表达及临床意义
- Author:
Li WEI
;
Lei WU
;
Junpeng JING
;
Yue WANG
;
Meng WU
;
Xue GONG
;
Zipeng LU
;
Jian XU
;
Fang WANG
;
Shiyang PAN
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
Antigens;
Tumor markers,biological;
Enzyme-linked immunosorbent assay;
Luminescent measurements
- From:
Chinese Journal of Laboratory Medicine
2016;39(4):277-280
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the changes of the NJ001 specific antigen expression before and after surgery, and evaluate whether the NJ001 specific antigen could be used as a serum biomarker for the diagnosis of pancreatic cancer.Methods With the method of sandwich ELISA, the serum samples from 85 pancreatic cancer patients, 22 pancreatic benign tumor and 40 healthy controls were detected respectively. The results of the NJ001 specific antigen in the serum samples from 85 pancreatic cancer patients were compared with CA19-9 detected by ECLIA.Results The positive rate of NJ001 for the pancreatic cancer group was obviously higher than that for the benign pancreatic tumor and health control groups[50.6%(43/85) vs 18.2%(4/22), χ2 =7.451, P<0.05; 50.6%(43/85) vs 10.0%(4/40), χ2 =19.098, P<0.05].The difference between benign pancreatic tumor group and health control group had no statistical significance[18.2%(4/22) vs 10.0%(4/40),χ2 =0.845, P>0.05].The positive rate in the group of pancreatic cancer before surgery was higher than that after surgery[50.6%(43/85) vs 23.5%(20/85),χ2=13.341, P<0.05].In addition, the results from 85 pancreatic cancer patients showed the specificity of NJ001 specific antigen was up to 87.1%.Although the positive rate of NJ001 specific antigen for pancreatic cancer was lower than that of CA19-9[50.6%(43/85) vs 75.3%(64/85), χ2 =11.121, P<0.05], it was higher when they combined [ 85.9%( 73/85 ) ] .Conclusions It shows high positive rate of NJ001 specific antigen in the patients of pancreatic cancer in this study, which suggests that NJ001 specific antigen might be a potential valuable biomarker for the diagnosis of pancreatic cancer.
