Direct identification of pathogens from positive blood cultures by matrix-assisted laser desorption ionization-time of flight mass spectrometry
10.3760/cma.j.issn.1674-2397.2016.02.011
- VernacularTitle:基质辅助激光解吸电离飞行时间质谱技术直接鉴定血流感染病原菌的效果评价
- Author:
Xiaofang XIE
;
Huiqin ZHOU
;
Yi ZHENG
;
Tingting LIU
;
Rongqin XU
;
Hong DU
- Publication Type:Journal Article
- Keywords:
Spectrometry,mass,matrix-assisted laser desorption-ionization;
Bacterial infection;
Bloodstream infection;
Diagnosis
- From:Chinese Journal of Clinical Infectious Diseases
2016;9(2):152-155
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a rapid and accurate technique for pathogens identification from positive blood cultures by matrix-assisted laser desorption ionization-time of flight mass spectrometry ( MALDI-TOF MS) .Methods A total of 266 culture-positive blood samples from clinical laboratory in the Second Affiliated Hospital of Soochow University during January to July 2015 were collected.The blood was transferred into separation-gel tubes, and the bacteria were enriched and purified by differential centrifugation.MALDI-TOF MS was applied to identify the bacteria and the results were compared with conventional bacterial culture. Results Among 266 culture-positive blood samples, 260 were monomicrobial cultures and 6 were polymicrobial cultures.Of 260 monomicrobial cultures, 98.8% (257/260) and 96.2% ( 250/260 ) of organisms were identified at the genus level and the species level, respectively.Of 140 Gram-negative bacterial isolates, 99.3% (139/140) and 97.9% (137/140) were identified at the genus level and the species level, respectively.Of 120 Gram-positive bacteria isolates, 98.3%(118/120) and 94.2% (113/120) were identified at the genus level and the species level, respectively.None of the 6 polymicrobial cultures were identified.Conclusion MALDI-TOF MS can directly identify the bacteria from positive blood cultures, which provides a rapid and accurate method for the diagnosis of bloodstream infection.
