Inhibitory effect of aminoglucomannan on human respiratory syncytial virus in vivo
10.3760/cma.j.issn.1674-2397.2016.02.015
- VernacularTitle:氨基葡甘露聚糖对人呼吸道合胞病毒的体内抑制作用
- Author:
Xiufeng SU
;
Na LI
;
Guomin CHEN
;
Xufang CHEN
- Publication Type:Journal Article
- Keywords:
Mannans;
Aminoglucomannan;
Respiratory syncytial viruses;
Antiviral agents
- From:
Chinese Journal of Clinical Infectious Diseases
2016;9(2):173-179
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the anti-human respiratory syncytial viurs ( hRSV ) effect of aminoglucomannan ( AGM) in mice.Methods BALB/c mice infected with hRSV were randomly divided into AGM and konjac glucomannan ( KGM) groups with 54 in each.Then, mice in AGM group and KGM group were subdivided into three groups and treated with different doses of AGM or KGM as 2.5 μmol/L, 0.25 μmol/L or 0.025μmol/L.Each subgroup was further divided into 3 groups with 6 in each according to administration intervals of AGM or KGM as 8 h, 12 h or 24 h.All the mice are sacrificed at 72 h.The general condition of the mice was observed everyday.Reverse transcription-polymerase chain reaction ( RT-PCR) was used to detect the expression of hRSV fusion protein ( F) mRNA in the lung tissue of the mice. HE staining method and immunohistochemistry technique for intercellular cell adhesion molecule-1 ( ICAM-1) expression in the lung tissue were used to evaluate the inflammation in the lung tissue.ANOVA for randomized block design was used to examine the influence of dose and administration intervals on the expressions of hRSV F mRNA and ICAM-1.Results Reduced activity and asthma were observed in 16 mice in the KGM group, but not in the AGM group.And two mice in the KGM group died at d3, but there was no death in the AGM group.RT-PCR showed that the levels of hRSV F mRNA in lung tissues were 0.49 ±0.21 in AGM group and 0.88 ±0.06 in KGM group (t=6.71, P<0.05).HE staining of lung tissue showed that the inflammation in KGM group was more serious than that in AGM group.The expression of ICAM-1 in AGM group was much lower than that in KGM group, and there was statistical difference between two groups (t=13.88, P<0.05).In AGM group, the levels of RSV F mRNA and ICAM-1 decreased with the increase of AGM’s concentration and rised with longer administration intervals (P<0.05), but these were not observed in KGM group (P>0.05).Conclusion AGM can alleviate the inflammation of lung tissue in mice infected with hRSV in a dose and time-depandent manner.
