Effects of Prdx4 protein expression on migration and invasion of HeLa cells
10.3969/j.issn.1000-4718.2016.04.010
- VernacularTitle:Prdx4蛋白表达改变对HeLa细胞迁移和侵袭的影响
- Author:
Weiyan YUAN
;
Li ZHANG
;
Hongqin SHI
;
Xiaowei GONG
;
Yong JIANG
- Publication Type:Journal Article
- Keywords:
Uterine cervical neoplasms;
Peroxiredoxins 4;
Cell migration;
Cell invasion
- From:
Chinese Journal of Pathophysiology
2016;32(4):637-643
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of peroxiredoxin 4 ( Prdx4) protein expression levels on the migra-tion and invasion of human cervical cancer HeLa cells.METHODS:The plasmid pcDNA3.0-HA-Prdx4 was transfected into HeLa cells.The HeLa cells were infected with LV-Prdx4 RNAi vector to establish stable Prdx4 shRNA HeLa cells. The change in the expression of Prdx4 protein was validated by Western blotting.The wound-healing assay, and Transwell migration and invasion assays were performed to detect the migration and invasion of HeLa cells, respectively.RESULTS:The expression of Prdx4 protein was up-regulated in the HeLa cells after transfection with pcDNA3.0-HA-Prdx4 plasmid ( P<0.05), whereas it was down-regulated in the Prdx4 shRNA HeLa cells (P<0.05).The abilities of migration and inva-sion were significantly increased in Prdx4-overexpressing HeLa cells compared with non-transfected and mock plasmid trans-fected control groups ( P<0.01) .When Prdx4 was knocked down by shRNA, the migration and invasion of the HeLa cells were remarkably repressed compared with blank control group and negative control group ( P<0.01 ) .CONCLUSION:The up-regulation of Prdx4 expression facilitates the migration and invasion of HeLa cells, and the down-regulation of Prdx4 expression inhibits the migration and invasion of HeLa cells, indicating that Prdx4 may be a potential molecular target for cervical cancer therapy.
