Effect of URG11 gene silencing on proliferation and invasion abilities of human prostate cancer cells
10.3969/j.issn.1000-4718.2016.04.013
- VernacularTitle:沉默URG11基因对前列腺癌LNCaP细胞增殖、迁移与侵袭的影响
- Author:
Bin PAN
;
Zhihai DENG
;
Youke WU
;
Weibo LIANG
;
Chuanrong ZENG
;
Haiping LIU
- Publication Type:Journal Article
- Keywords:
Up-regulated gene 11;
Prostate cancer;
RNA interference;
Neoplasm invasion
- From:
Chinese Journal of Pathophysiology
2016;32(4):658-664
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the expression of up-regulated gene 11 ( URG11 ) in prostate cancer cell line and the effect of URG11 siNRA on the proliferation and invasion of human prostate cancer LNCaP cells.METHODS:The mRNA and protein levels of URG11 in prostate cancer cell lines and normal prostate epithelial cell line were evaluated by real-time PCR and Western blot.LNCaP cells were transfected with designed siRNA using the liposome method.The prolif-eration, apoptosis, migration and invasion abilities of the LNCaP cells were evaluated by MTS assay, flow cytometry, wound-healing assay and Transwell assay.RESULTS:The expression of URG11 at mRNA and protein levels in the DU145, PC3, LNCaP cell lines was significantly higher than that in RWPE-1 cell line.Compared with the control group, the proliferation of LNCaP cells with URG11 siRNA was stagnant in G1/S phase and induced apoptosis.The proliferation of LNCaP cells at 0 h, 24 h, 48 h and 72 h was inhibited after URG11 expression was down-regulated (P<0.05).Transwell assay showed that migration (P<0.05) and invasion (P<0.05) were also inhibited.CONCLUSION:URG11 is highly expressed in prostate cancer.Silencing of URG11 significantly inhibits the proliferation and invasion and induces apoptosis of LNCaP cells.
