Role of hydrogen sulfide in ileal epithelial cell apoptosis by MAPK signa-ling pathway in rats with intestinal ischemia-reperfusion injury
10.3969/j.issn.1000-4718.2016.04.018
- VernacularTitle:H2S通过MAPK信号通路影响缺血再灌注损伤大鼠回肠上皮细胞凋亡
- Author:
Genlin LU
;
Aibing WU
;
Hongbin WANG
- Publication Type:Journal Article
- Keywords:
Ischemia-reperfusion injury;
Hydrogen sulfide;
MAPK signaling pathway;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2016;32(4):691-695
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of hydrogen sulfide (H2S) on the expression of MAPKs and ileal epithelial cell apoptosis in the rats with intestinal ischemia-reperfusion ( I/R) injury.METHODS:Healthy female Wistar rats were randomly divided into sham operation group, I/R group, I/R+sodium hydrosulfide ( NaHS) group .The animal model of intestinal ischemia reperfusion was established.Apoptosis index ( AI) of ileal epithelial cell was measured by TUNEL assay.H2 S was detected by sensitive sulfide electrode.The mRNA expression of ERK, JNK and p38MAPK was detected by RT-PCR.The protein levels of phosphorylation( p)-ERK, p-JNK, p-NF-κB P65 and p38MAPK were deter-mined by Western blot.RESULTS:H2 S, ERK mRNA and p-ERK in I/R group were significantly higher than those in I/R+NaHS group and sham group while JNK mRNA, p38MAPK mRNA, p-JNK, p-p38MAPK, p-NF-κB P65 and AI were predominantly higher than those in I/R+NaHS group and sham group (P<0.01).CONCLUSION:H2S attenuates ileal epithelial cell apoptosis in the rats with intestinal I/R injury by down-regulating ERK mRNA p-ERk and up-regulating JNK mRNA, p38MAPK mRNA and phosphorylation of JNK, p38MAPK and NF-κB P65.
