Effect of targeted inhibition of occludin by microRNA-429 on intestinal epithelial barrier function in diabetic mouse
10.3969/j.issn.1000-4718.2016.04.019
- VernacularTitle:M icroRNA-429靶向抑制occludin及对糖尿病小鼠肠上皮屏障功能的影响
- Author:
Xiji LU
;
Tao YU
;
Zhongsheng XIA
;
Tidong SHAN
;
Hui OUYANG
;
Jihao XU
;
Qikui CHEN
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus;
microRNA-429;
Occludin;
Intestinal epithelial barrier function
- From:
Chinese Journal of Pathophysiology
2016;32(4):696-700
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of microRNA-429 (miR-429) on the expression of occludin (Ocln) in intestinal epithelial cells ( IECs) and intestinal epithelial barrier function in diabetic mice.METHODS:Diabetes mel-litus mouse model was induced by intraperitoneal injection of streptozocin.The expression of miR-429 in IECs of diabetic mice was inhibited by antagomiRNA-429 injected via tail vein.The expression of miR-429 and mRNA expression of Ocln were detected by real-time PCR.The protein expression of Ocln was determined by Western blotting and immunohistochem-istry.The urinary lactulose/mannitol ratio was measured by gas chromatography.The plasma LPS concentrations in the mice were measured by chromogenic end-point TAL kit.RESULTS:The results of real-time PCR confirmed that the ex-pression of miR-429 in IECs of diabetic mice was remarkably inhibited by tail-vein administration of antagomiRNA-429, and resumed to similar level of normal mice on the 6th day after the administration.After suppressing the level of miR-429, the expression of Ocln in IECs of diabetic mice increased significantly (P<0.05), while the urinary lactulose/mannitol ra-tio and the plasma LPS concentration decreased obviously ( P<0.05 ) .CONCLUSION:AntagomiRNA-429 effectively suppresses miR-429 expression in IECs of diabetic mice, and then enhances the expression of Ocln and partially resumes the intestinal epithelial barrier function.
