Recombinant adenovirus containing hyper-interleukin-6 and hepatocyte growth factor enhances therapeutic efficacy on acute-on-chronic liver fai-lure in rats
10.3969/j.issn.1000-4718.2016.04.021
- VernacularTitle:HIL-6和HGF双基因重组腺病毒增强对慢加急性肝衰竭大鼠的治疗效果
- Author:
Dandan GAO
;
Jia FU
;
Bo QIN
;
Wenxiang HUANG
;
Chun YANG
;
Bei JIA
- Publication Type:Journal Article
- Keywords:
Acute-on-chronic liver failure;
Hyper-interleukin-6;
Hepatocyte growth factor;
Recombinant ade-novirus;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2016;32(4):707-712
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To determine the therapeutic efficacy of recombinant adenovirus containing hyper-interleu-kin-6 (HIL-6) and hepatocyte growth factor (HGF) (Ad-HGF-HIL-6) on acute-on-chronic liver failure (ACLF) in rats u-sing that of recombinant adenovirus HIL-6 or HGF ( Ad-HIL-6 or Ad-HGF) for comparison.METHODS:The rat model of ACLF was established and the model rats were randomly divided into model group, Ad0 group, Ad-HGF group, Ad-HIL-6 group and Ad-HGF-HIL-6 group.The sera and liver tissues were collected for biochemical, pathological and molecular bio-logical examinations.RESULTS:Compared with Ad0 group, prothrombin time ( PT) and the serum levels of alanine amin-otransferase (ALT), tumor necrosis factor-α(TNF-α), interferon-γ(IFN-γ) and high-mobility group box-1 (HMGB1) were markedly reduced in the ACLF rats treated with Ad-HGF, Ad-HIL-6 and Ad-HGF-HIL-6, and similarly, reduced he-patic damages and apoptotic activity, reduced Bax at protein level, and increased expression of Ki67 and Bcl-2 at protein levels were observed.Among them, treatment with Ad-HGF-HIL-6 showed the most significant therapeutic efficacy without obvious side effects.CONCLUSION:The therapeutic efficacy of Ad-HGF-HIL-6 is more potent than that of Ad-HGF or Ad-HIL-6 alone on ACLF rats with no significant side effects.
