Effect of Ca2+-activated Chloride Channel Density on Gating Properties of Anoctamin 1
10.12007/j.issn.0258-4646.2016.04.003
- VernacularTitle:钙激活氯通道密度调节Anoctamin 1电流作用的研究
- Author:
Ke MA
;
Hui WANG
;
Qinghua WANG
;
Shuya LUO
;
Qinghuan XIAO
- Publication Type:Journal Article
- Keywords:
Anoctamin1;
Ca2+-activated chloride channel;
channel density;
activation kinetics;
tumor
- From:
Journal of China Medical University
2016;45(4):298-300
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of channel density on the gating properties of Anoctamin 1(Ano1,TMEM16A)Ca2+?activated chlo?ride channel. Methods Ano1 expression plasmids were transiently transfected into HEK293 cells. High density and low density of Ano1 was ob?tained after expressing the protein for 24 h and 6 h,respectively. Electrophysiological recordings were performed in the whole?cell patch clamp con?figuration. The activation kinetics of current traces was fitted by exponentials. Results The current density was significantly higher in cells express?ing Ano1 for 24 h than those expressing Ano1 for 6 h(P<0.05). The activation of Ano1 current in cells with low density was well fit by a single expo?nential withτslow of 292.71±38.11 ms. The activation of Ano1 current in cells with high density was well fit by two exponentials withτfast of 47.78±4.58 ms andτslow of 385.74±71.44 ms. ANO1 current in cells with high density has a rapid active component(τfast)more than low density. There was no significantly different of theτslow between cells with high density and low density of Ano1(P>0.05). Conclusion Our findings suggested that chan?nel density regulates the gating of Ano1. High channel density promotes activation of Ano1.
