Antimicrobial resistance and mechanisms of ceftriaxone resistance in clini-cally isolated nontyphoidal Salmonella
10.3969/j.issn.1671-9638.2016.04.001
- VernacularTitle:临床分离非伤寒沙门菌耐药性及对头孢曲松耐药机制
- Author:
Wei SHI
;
Xiaoxia LIU
;
Shuangyu HAN
;
Jie YANG
;
Zhan ZHAO
;
Yubao WANG
- Publication Type:Journal Article
- Keywords:
nontyphoidal Salmonella;
multilocus sequence typing;
pulsed-field gel electrophoresis;
extended-spectrumβ-lactamase;
fluoroquinolone;
drug resistance;
microbial;
drug resistance
- From:
Chinese Journal of Infection Control
2016;15(4):217-221
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate antimicrobial resistance and ceftriaxone resistance mechanisms in clinically isolated nontyphoidal Salmonella(NTS),and provide evidence for the prevention and control of NTS infection and rational use of antimicrobial agents.Methods 108 NTS isolates were isolated from stool specimens of outpatients with acute diarrhea in the Second Hospital of Tianjin Medical University and Tianjin Medical University General Hospital from May to October of 2014,NTS were performed antimicrobial susceptibility testing;non-ceftriaxone-susceptible isolates were typed by serological,multilocus sequence (MLST),and pulsed-field gel electrophoresis (PFGE)methods,extended-spectrumβ-lactamase (ESBL)detection and AmpC genes were detection.Results Among 108 NTS isolates,mono-drug resistance rate to 11 antimicrobial agents was 49.07% (n= 53),multidrug resistance rate was17.59% . Susceptibility rates to nalidixic acid,levofloxacin,ciprofloxacin,ceftriaxone,and ertapenem were 61.11% ,66.67% ,68.52% ,97.22% ,and 100.00% respectively. Three non-ceftriaxone-susceptible NTS isolates were detected,2 were ST11 Salmonellaenterica serotype (Sa8709,Sa8771),1 was ST34 Salmonellatyphimurium serotype(Sa8763). Cluster analysis of PFGE revealed that Sa8709 was highly similar to Sa8771 strains(91 .70% ), but the similarity to Sa8763 was low(55.80% );Sa8709 strain carried CTX-M gene,Sa8771 strain carried CTX-M and TEM genes,Sa8763 strain carried OXA gene. Conclusion Clinically isolated NTS in this area are low resistant to fluoroquinolones,multidrug resistant strains carrying ESBLs have emerged.
