Clinical Significance and Functional Prediction of Up-regulated RAD21 in Lung Cancer
10.12007/j.issn.0258-4646.2016.04.010
- VernacularTitle:肺癌中异常增高的RAD21的临床意义及潜在功能预测
- Author:
Xuejing GU
;
Yueming WU
;
Jing GAO
- Publication Type:Journal Article
- Keywords:
lung cancer;
RAD21;
prognosis;
immunohistochemistry
- From:
Journal of China Medical University
2016;45(4):328-332
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression levels of RAD21(S.Pombe RAD21 homolog)in lung cancer,and assess its clinical significance and potential functions. Methods The expression level of RAD21 in lung cancer was determined by Western blot and immunohistochemistry meth?ods;the RAD21 mRNA expression in lung cancer was analyzed through Oncomine database;the relationship between the RAD21 expression levels and prognosis in patients with lung cancer was studied by Kaplan Meier curve;the RAD21 functions in lung cancer were predicted by using gene set enrichment analysis. Results Compared with adjacent normal tissue,the protein level of RAD21 in lung tissues were upregulated . The data from Oncomine database showed that RAD21 mRNA levels were significantly higher than the normal control groups in multiple lung cancer datasets(P<0.01);in addition,Kaplan?Meier survival curves showed that the RAD21 expression levels was correlated with the survival of patients with lung can?cer(P<0.05);gene set enrichment analysis indicated that RAD21 was mainly enriched in apoptotic signaling pathways and cell cycle regulation gene sets. Conclusion The downregulated RAD21 could be used as a biomarker to assess the prognosis of lung cancer patients. In addition, RAD21 may be involved in the regulation of cell cycle and apoptosis,which affects the development and progression of lung cancer.
