G-quadruplex Structures in Promoters of MET Proto-oncogene
10.12007/j.issn.0258-4646.2016.05.005
- VernacularTitle:原癌基因MET启动子的G-四链体结构
- Author:
Jing YAN
;
Yifu GUAN
- Publication Type:Journal Article
- Keywords:
G-quadruplex;
MET proto-oncogene;
regulation of gene expression
- From:
Journal of China Medical University
2016;45(5):402-405
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify G?quadruplex structures in the promoter region of MET. Methods CD spectroscopy,UV spectroscopy,non?denatured electrophoresis and PCR stop assay were applied to indicate the G?quadruplex structure and its function. Results The Pu23WT se?quence in the promoter of MET adopted an intramolecular parallel G?quadruplex structure under physiological conditions in vitro,which can stop the extension of Pmet. Conclusion G?quadruplex structure in the promoter might inhibit MET gene expression in vivo.
