IFN-αcould induce the expression of SAMHD1 by down-regulating miR-181a

VernacularTitle:IFN-α可以通过抑制miR-181 a水平间接调控SAMHD1的表达
Author: Heping RAO ; Wei WANG ; Xiangning JIN ; Changzhong JIN
Publication Type:Journal Article
Keywords: SAMHD1; IFN-α; miR-181a; HIV infection; Inflammatory response
From: Chinese Journal of Microbiology and Immunology 2016;36(4):277-280
CountryChina
Language:Chinese
Abstract: Objective To investigate whether the expression of sterile alpha motif and histidine/aspartic acid domain containing protein 1 ( SAMHD1 ) could be induced by IFN-α and mediated by microRNA-181a (miR-181a). Methods THP-1 and Jurkat cells were treated with different doses of IFN-α(200 IU/ml and 1 000 IU/ml) for 24 h. The expression of miR-181a and SAMHD1 at mRNA level were de-tected by quantitative PCR. Western blot assay was performed to measure the expression of SAMHD1 at pro-tein level. THP-1 and Jurkat cells were transfected with p-181a, an over-expression vector of miR-181a, and then treated with 200 IU/ml of IFN-α. Changes in the expression of SAMHD1 in those cells were analyzed. Results IFN-α significantly enhanced the expression of SAMHD1 at mRNA and protein levels, but inhibi-ted the expression of miR-181a, especially in Jurkat cells. The expression of SAMHD induced by IFN-αwas inhibited in cells over-expressing miR-181a. Conclusion This study suggests that IFN-α could induce the expression of SAMHD1 by down-regulating the level of miR-181a.