Effect of CTGF siRNA on Apoptosis of Fibroblast-like Synoviocytes of Rheumatoid Arthritis
10.12007/j.issn.0258-4646.2016.05.012
- VernacularTitle:CTGF siRNA对类风湿关节炎成纤维样滑膜细胞凋亡的影响
- Author:
Shuang DING
;
Fang FANG
;
Hongmei DUAN
;
Weiguo XIAO
- Publication Type:Journal Article
- Keywords:
connective tissue growth factor;
fibroblast-like synoviocytes;
apoptosis
- From:
Journal of China Medical University
2016;45(5):430-433
- CountryChina
- Language:Chinese
-
Abstract:
Objective To silence the expression of CTGF by small interfering RNA technology,to observe the influence on fibroblast?like synovial cell apoptosis and several apoptosis?related genes,and to explore the mechanism of action of CTGF in rheumatoid arthritis synovial lesions. Methods Effective CTGF siRNA was screened through real?time PCR. The influence of CTGF siRNA on FLS apoptosis was detected with FITC?PI double staining by flow cytometry. bax,bcl?xl and survivin were detected using real?time PCR when CTGF mRNA has been silenced. Results Compared with other 2 groups of oligo and NC oligo,H1 oligo exhibited the strongest interfering action to CTGF(inhibition ratio>70%),so that it is selected as the effective target gene sequence for the following experiment. Apoptosis of FLS induced by serum deprivation was significantly decreased in the presence of exogenous CTGF. When expression of the CTGFgene was knocked down in FLS,FLS apoptosis was significantly increased,and expres?sion levels of survivin mRNA were decreased significantly(P<0.01). Conclusion FLS survival is positively regulated by CTGF,which may through the sustaining the expression of survivin.
