Application of ELISA kit for the detection of hepatitis E virus antigen in plasma donations
10.3760/cma.j.issn.0254-5101.2016.04.013
- VernacularTitle:戊型肝炎抗原检测试剂在供血浆人群的应用
- Author:
Weijin HUANG
;
Aijing SONG
;
Shan QIAO
;
Chenyan ZHAO
;
Xuerong JIA
;
Yan ZHANG
;
Youchun WANG
- Publication Type:Journal Article
- Keywords:
Hepatitis E virus(HEV);
Antigen;
RNA;
Plasma
- From:
Chinese Journal of Microbiology and Immunology
2016;36(4):300-304
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the practicability of using ELISA kit for the detection of hepati-tis E virus antigen ( HEV-Ag) in plasma donations and Biomex HEV seroconversion panels. Methods The HEV-Ag positive samples were screened out from 36 340 donated blood plasma samples. Real-time fluores-cent PCR was performed for the detection of HEV RNA in HEV-Ag positive samples. The open reading frame 2 (ORF2) in HEV RNA was amplified by nested RT-PCR and the amplified products were confirmed by sequencing analysis. Phylogenetic tree was constructed for HEV genotyping. Five Biomex HEV serocon-version panels were used in this study for the detection of HEV-Ag, anti-HEV antibody and HEV RNA as well as the correlation analysis between HEV-Ag and HEV RNA. Results Twenty-six out of 36 340 plasma samples (0. 07%) were positive for HEV-Ag. Of the 26 samples, 25 samples were positive for HEV RNA as indicated by the results of nested RT-PCR and 23 positive samples were confirmed by sequencing analysis. The positive rate of HEV RNA in blood plasma donators was 1 ∶ 1 580 (0. 06%). There were 17 samples of genotype 1 (74%) and 6 samples of genotype 4 (26%) according to the phylogenetic tree analysis. All of the HEV-Ag positive samples were also positive for HEV RNA as indicated by the analysis of Biomex sero-conversion panels. HEV-Ag was consistent with the peak of the HEV RNA concentration. Conclusion A close relationship between HEV-Ag and HEV RNA was observed. HEV-Ag screening could be used as a measure to reduce the risk of HEV transmission by blood transfusion.
