Expression of YAP in human papillary thyroid cancer and its influence on cell growth
10.3969/j.issn.1671-8348.2016.12.022
- VernacularTitle:YAP在人甲状腺乳头状癌中的表达及对细胞生长的影响
- Author:
Jie ZHANG
;
Yi LIN
;
Ya LIN
- Publication Type:Journal Article
- Keywords:
Yes-associated protein;
carcinoma,papillary;
thyroid neoplasms;
gene expression;
cell proliferation
- From:
Chongqing Medicine
2016;45(12):1653-1655
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of Yes‐associated protein(YAP) in human papillary thyroid cancer and its influence on cell growth .Methods The samples in 57 cases of papillary thyroid cancer treated by operation resection in the gen‐eral surgery department of this hospital and the matched tumor‐adjacent tissues were collected .All the cases were definitely diag‐nosed by the pathology examination .The expression of YAP protein in the cancer tissue and corresponding tumor‐adjacent tissue were determined by the immunohistochemistry(IHC)staining .The relationship between the YAP protein and the clinicopathological data was statistically analyzed .siRNA was used to silence the expression of YAP in B‐CPAP cells ,MTT and flow cytometry were used to measure the proliferation and apoptosis changes .Results The expression level of YAP was markedly higher in papillary thyroid cancer tissues than in tumor adjacent tissues (P<0 .05);moreover the expression of YAP protein was positively correlated with the tumor size and TNM stage (P<0 .05) .Silencing YAP gene could significantly inhibit the cell proliferation ability and pro‐mote cell apoptosis(P<0 .05) .Conclusion YAP is highly expressed in papillary thyroid cancer tissues and is related with its ad‐verse clinicopathological characteristics ,down‐regulating YAP gene can significantly inhibit the cell growth .
