Effects of Electroacupuncture on Expression of Glutamate Receptor Interacting Proteins 1 and 2 in Hippocampus of Rats with Alzheimer's Disease
10.3969/j.issn.1006-9771.2016.04.009
- VernacularTitle:电针对阿尔茨海默病大鼠海马谷氨酸受体相互作用蛋白1、2表达的影响
- Author:
Ruolan LIU
;
Chen SHEN
;
Xiaoyan SHEN
;
Jun TIAN
- Publication Type:Journal Article
- Keywords:
Alzheimer's disease;
electroacupuncture;
glutamate receptor interacting protein;
rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2016;22(4):413-416
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of electroacupuncture on the expression ofα-amino-3-hydroxyl-5-methyl-4-isoxazole propionate (AMPA) receptor associated protein glutamate receptor interacting protein (GRIP)1 and GRIP2 in hippocampal neurons of rats with Alzheimer's disease (AD) model induced by Aβ25-35. Methods 40 Male Sprague-Dawley rats were randomly divided into normal group, sham operation group, model group and electroacupuncture group with 10 rats in each group. The AD rat model was prepared by injecting Aβ25-35 in the hippocampus CA1 of rats, while the sham operation group was injected with equal amount of normal saline at the same loca-tion. On the second day after successful modeling, the electroacupuncture group received electroacupuncture at Baihui (DU20) and bilateral Shenshu (BL23) acupoints, once a day, 6 times a week for 2 weeks. The expression of GRIP1 and GRIP2 were detected with immunohisto-chemistry. Results There was no difference in the expression of GRIP2 and GRIP1 proteins in hippocampus between the normal group and sham operation group (t<1.7438, P>0.05), but was lower in the model group and the electroacupuncture group than in the sham operation group (t>9.5928, P<0.001), and was higher in the electroacupuncture group than in the model group (t>9.5326, P<0.05). Conclusion Elec-troacupuncture may increase the number of AMPA receptors on the postsynaptic membrane by increasing GRIP1 and GRIP2.
