Hyperbaric Oxygen Induced Autophagy in Neuropathic Rats

VernacularTitle:高压氧诱导神经病理性疼痛大鼠自噬的作用
Author: Guang HAN ; Lu LI ; Ping ZHAO
Publication Type:Journal Article
Keywords: hyperbaric oxygen; chronic constriction injury; the sciatic nerve; P62; microtubulerassociated protein 1 light chain 3; mi-tochondria; autophagy
From: Journal of China Medical University 2016;45(4):349-351
CountryChina
Language:Chinese
Abstract: Objective To investigate whether hyperbaric oxygen(HBO)affected neuropathic pain through autophagy and verify the HBO effect. Methods A total of 60 male SD rats were selected for the study and randomly divided into 4 groups:control group(C group),sham operation group (S group),chronic constriction group(CCI group)and hyperbaric oxygen group(HBO group). LC3Ⅱand P62 were determined by Western blot method and the autophagy was detected by electron microscopy on the 7th day. Results CCI and HBO groups were significantly higher than C and S group in Western blot(P<0.05). There was no difference between group C and group S(P>0.05). The expression of LC3Ⅱin HBO group was significantly higher than that in CCI group(P<0.05). And the expression of P62 in CCI group was significantly higher than that in HBO group(P<0.05). The autophagy in CCI and HBO group were significantly increased comparing to the C and S groups(P<0.05). However,the difference be?tween group C and S was not significant(P>0.05). Conclusion Hyperbaric oxygen therapy can induce autophagy for anti?neuropathic pain.