Expression of Shank1 and its clinical significance in renal cancer tissue
10.3969/j.issn.1000-8179.2016.08.064
- VernacularTitle:Shank1在肾细胞癌组织中的表达及临床意义
- Author:
Wanli ZHAO
;
Sheng LIU
;
Min LIU
;
Xudong ZHOU
;
Pengyu SUN
;
Xian WANG
;
Jihong YANG
;
Xiaoli MA
- Publication Type:Journal Article
- Keywords:
renal cell carcinoma;
Shank1;
protein expression;
immunohistochemistry
- From:
Chinese Journal of Clinical Oncology
2016;43(8):329-333
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect the expression of Shank1 protein in renal cell carcinoma (RCC), to investigate its difference between the tumor and carcinoma adjacent tissue, and to analyze its correlation with RCC clinicopathological characteristics and prognosis. Methods:The renal carcinoma and carcinoma adjacent tissues of 120 patients were selected from Cangzhou Central Hospital and Ji'nan Central Hospital from May 2008 to December 2014. The expression level of Shank1 was determined by immunohistochemistry and Western blot. Statistical analysis was performed to determine the relationship between the expression of Shank1 and the clinicopathological features of RCC patients. Results:Results of immunohistochemistry showed that the expression level of Shank1 in renal cancer tissue was significantly higher than that in carcinoma adjacent tissue, and the difference was statistically significant (P<0.05). Western blot results showed that the expression level of Shank1 protein in renal cancer tissue was also significantly higher than in carcinoma adjacent tissue. Correlation analysis found that the high expression level of Shank1 in renal cancer tissue was not significantly related to gender, age, tumor size, and TNM stage, but was significantly associated with the histological differentiation of RCC (P<0.05). Conclusion:Shank1 is abnormal y expressed in RCC renal cancer tissues and is correlated with the histological differentiation of RCC.
