The effects of cisplatin on cancer stem cell markers of hepatocellular carcinoma cell line HepG2
10.3969/j.issn.1673-4130.2016.08.003
- VernacularTitle:顺铂作用于人肝癌细胞系HepG2后对肿瘤干细胞标志物的影响
- Author:
Yingjia XU
;
Yinjun XUE
;
Chanjuan LU
- Publication Type:Journal Article
- Keywords:
cisplatin;
liver cancer;
cancer stem cells;
cell proliferation;
cell cycle
- From:
International Journal of Laboratory Medicine
2016;37(8):1023-1025
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of cisplatin on the expression of tumor stem cell markers in human hepatoma cell line HepG2 .Methods The cultured human hepatoma HepG2 cells in logarithmic growth phase were used for the study ,which were divided into control(no special treatment was administrated) and experimental groups(cisplatin was added) ,then the rates of cell growth inhibition ,transitional healing rates and changes in tumor associated markers associated P53 ,caspase‐3 ,caspase‐8 ,CD133 ,a‐denosine triphosphate‐binding cassette superfamily G member 2(ABCG2) ,intercellular cell adhesion molecule‐1(ICAM‐1) were measured .Results Comparison of cell growth inhibition :with the increase of drug concentration increased ,compared with the con‐trol group ,the difference was statistically significant(P<0 .05) .Transitional healing rate:with the increase of drug concentration , compared with the control group ,the difference was statistically significant(P< 0 .05) .The expression of related tumor markers P53 ,caspase‐3 ,caspase‐8 increased while the drug concentrations increase and were higher in the control group(P< 0 .05) ,the difference were statistically significant .In the immunofluorescence for the detection of CD133 ,ABCG2 ,ICAM‐1 ,the fluorescence in‐tensity increased with the concentrations of cisplatin .Conclusion Cisplatin could inhibit the growth of tumor cells by up‐regulating the tumor markers such as P53 ,caspase‐3 and caspase‐8 .The migration of tumor cells in cell scratch test could also be inhibited by cisplatin w hile their drug resistance w ere enhanced .
