Serotyping and genotyping study of clinical Vibrio parahaemolyticus

VernacularTitle:临床副溶血弧菌血清型及基因分型特征研究
Author: Xiaojing YIN ; Wei XU ; Dongsheng HAN
Publication Type:Journal Article
Keywords: vibrio parahaemolyticus; serotyping; virulence; genotyping
From:International Journal of Laboratory Medicine 2016;37(8):1028-1029
CountryChina
Language:Chinese
Abstract: Objective To study the distribution and molecular characteristics of Vibrio parahaemolyticus(VP) in patients with a‐cute diarrhea ,and provide a scientific basis for the prevention and control of VP infection .Methods From 2010 to 2014 ,62 VP iso‐lates were collected from patients with acute diarrhea ,for serotyping and virulence gene (tdh and tdh) detection of VP .Molecular characteristics analysis was carried out by using multi‐locus sequence typing (MLST) .Results 7 different serotypes were found from the 62 isolates .O3∶K6 was the most common serotype of VP ,accounting for 74 .19% (46 isolates) ,followed by O4∶K68 (6 isolates) .Tdh gene was the mainly virulence gene ,with a percentage of 95 .16% (59 isolates) ,only three isolates were trh positive . 7 STs were found through MLST analysis of 62 VP isolates ,among which ,ST3 was the most important type ,accounted for 85 .50%(53 isolates) .Conclusion O3∶ K6 serotype VP was the most prevalent type .Tdh gene is the most important virulence gene of WP .ST3 was the the dominant epidemic type .