Expression and significance of p38 mitogen-activated protein kinase in oral lichen planus and oral squamous cell cacinoma
10.3969/j.issn.1671-167X.2016.02.024
- VernacularTitle:p38丝裂原活化蛋白激酶在口腔扁平苔癣及口腔鳞状细胞癌中的表达及意义
- Author:
Xiaosheng HU
;
Yunhui HUANG
;
Xiaosong LIU
;
Hong HUA
- Publication Type:Journal Article
- Keywords:
p38 mitogen-activated protein kinases;
Lichen planus,oral;
Carcinoma,squamous cell;
NF-kappa B
- From:
Journal of Peking University(Health Sciences)
2016;48(2):310-315
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To assess the differences among the expressions of p38 mitogen activated protein kinases (MAPK),phospho-p38MAPK and nuclear factor kappa B (NF-κB)in oral lichen planus (OLP)and oral squamous cell carcinoma (OSCC ).Methods:In the study,53 cases of OLP,45 of OSCC,and 18 controls were obtained and 4-μm-thick histological sections were prepared from formalin-fixed paraffin-embedded tissue blocks.The expressions of p38MAPK,phospho-p38MAPK and NF-κB were detected by immunohistochemistry staining.Furthermore,the expressions of p38MAPK and phospho-p38MAPK were detected using Western blotting analyses in the fresh tissues from 1 1 cases of OLP,5 ca-ses of OSCC,and 7 cases of the controls.Results:p38MAPK was over-expressed in the lamina propria, but lowly expressed in the epithelium in OLP group.Phospho-p38MAPK was lower expressed in OLP group than in OSCC and control groups.NF-κB was found over-expressed in the lamina propria in OLP group.p38MAPK was found expressed in all the samples in the 3 groups.The expression of phospho-p38MAPK was observed in 8 (8/11)OLP samples,5 (5/5)OSCC samples and 4 (4/7)controls by Western blotting,but no significant differences were found within the 3 groups.Conclusion:p38MAPK can be detected in normal oral mucosa,OLP and OSCC.phospho-p38MAPK may be related to the onset and progression of OSCC.The role of p38MAPK in OLP is yet to be revealed.
