Role of CHOP pathway in ischemia-reperfusion induced lung injury in mice and its molecular mechanism
10.3969/j.issn.1005-1678.2016.04.11
- VernacularTitle:CCAAT／增强子结合蛋白同源蛋白在小鼠肺缺血再灌注损伤中的作用机制
- Author:
Zhenzhen WANG
;
Xinjian DAI
;
Jiyang ZHENG
;
Wantie WANG
- Publication Type:Journal Article
- Keywords:
lung;
reperfusion injury;
CHOP;
apoptosis
- From:
Chinese Journal of Biochemical Pharmaceutics
2016;36(4):45-48
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role of CCAAT/enhancer-binding protein homologous protein (CHOP) in ischemia-reperfusion induced lung injury, and clarify the potential molecular mechanism.Methods Fifty mice of C57BL/6J were randomly divided into five groups, 10 mice in each group, including Sham operation group(sham group),ischemia/reperfusion group (I/R group), I/R +PBS+Lipofectamine group(I/R+PBS+Lipo group) , I/R+scramble siRNA group( I/R+siRNASCR group) , I/R+CHOPsiRNA group( I/R+siRNACHOP group).The content of total lung water (TLW), wet-to-dry weight ratio (W/D) of lung and index of quantitative assessment of histologic lung injury (IQA) were detected, CHOP mRNA and protein expression were detected by RT-PCR and Western blot.Results Compared with Sham group, W/D, TLW and IQA were significantly elevated in I/R group,I/R+PBS+Lipo group and I/R+siRNASCR group (P<0.05).Moreover, the W/D,TLW and IQA reduced with CHOP-siRNA treatment, respectively(P<0.01).Compared with Sham group, CHOP mRNA and protein expressions were significantly elevated in I/R group,I/R+PBS+Lipo group and I/R+siRNASCR group, Moreover, the CHOP mRNA and protein expressions reduced with CHOP-siRNA treatment, respectively(P<0.01). Conclusion I/R could cause excessive unfolded protein response in lung tissue, and induce apoptosis by CHOP signal pathway, damage lung tissue. The inhibition of CHOP pathway could alleviate lung ischemia-reperfusion injury.
