Regulative effect of collagen and related protease gene of betulin on human ESF-1 cells
10.3760/cma.j.issn.1673-4246.2016.05.011
- VernacularTitle:白桦脂醇对人皮肤成纤维细胞胶原蛋白及相关蛋白酶基因的调控机制研究
- Author:
Lihong ZHANG
;
Hui XIONG
;
Zhonghua HU
;
Guoliang LIU
;
Hongdan XU
;
Ning ZHANG
;
Jianmin LI
- Publication Type:Journal Article
- Keywords:
Skin aging;
Betulin;
Collagen;
Matrix metalloproteinases
- From:
International Journal of Traditional Chinese Medicine
2016;38(5):420-423
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism and regulative function of betulin on anti-aging genes related of normol human dermal fibroblasts (ESF-1), and to reveal the repairing mechanism of betulin on wrinkles. Methods The cultured cell in vitro were divided into the control group, the estradiol group and the betulin high, medium and low dose groups. Competence of ESF-1 cells was detected by MTT. Expression of mRNA of collagen type I and Ⅲ (ColⅠand Col Ⅲ), tissue inhibitors of matrix metalloproteinase 1 and 2 (TIMP-1 and TIMP-2), matrix metalloproteinase 1 (MMP-1) was detected by RT-PCR. Results Compared with the control group, the proliferation of ESF-1 cells (0.455 ± 0.037, 0.445 ± 0.040 vs. 0.385 ± 0.601) in the estradiol group and the medial-dose betulin group were increased (P<0.05). Compared with the control group, the expression of mRNA of ColⅠ (0.960 ± 0.012, 0.929 ± 0.015 vs. 0.842 ± 0.014), Col Ⅲ (0.892 ± 0.009, 0.824 ± 0.022 vs. 0.768 ± 0.025), TIMP-1 (0.938 ± 0.026, 0.878 ± 0.035 vs. 0.796 ± 0.022), TIMP-2 (0.557 ± 0.025, 0.506 ± 0.036 vs. 0.436 ± 0.063) in the estradiol group and the medial-dose betulin group were increased (all P<0.01). Conclusion Betulin based on increasing the competence of ESF-1 cells, promoting collagen synthesis and the expression level of related-proteinase is to suspend the development of wrinkles.
