Determination of SBGC and Hyperin in the Effective Fraction of Jiangzhining
10.3870/j.issn.1004-0781.2016.05.022
- VernacularTitle:降脂宁有效部位中芪多酚和金丝桃苷的含量测定
- Author:
Juan CAO
;
Fugang WANG
;
Ke LIU
;
Huiling LI
- Publication Type:Journal Article
- Keywords:
Jiangzhining;
Chromatography,high performance liquid;
2,3,5,4 ’-Tetrahydroxystibene-2-O-β-D-Glucoside;
Hyperin;
Determination
- From:
Herald of Medicine
2016;35(5):518-520
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method for determination of 2,3,5,4’-tetrahydroxystibene-2-O-β-D-glucoside(SBGC)and hyperin in the effective fraction of Jiangzhining. Methods HPLC analysis was performed on a C18 column(4.6 mm×250 mm,5 μm),with acetonitrile-0.1% methanoic acid water solution(15.5: 84.5)serving as the mobile phase. The flow rate was 1.0 mL·min-1 ,the wavelength was 340 nm,and the injection volume was 20 μL. Results The calibration curve was linear for SBGC in the range of 24-120 μg·mL-1(r = 0.999 8)and for hyperin in the range of 2.4-12.0 μg·mL-1(r= 0.999 6),respectively.Their average recoveries were 100.07% and 100.14%,respectively.The contents of SBGC and hyperin were 2.26% and 0.23%,respectively Conclusion The method is convenient,precise and reliable for determination of the content of SBGC and hyperin in the effective fraction of Jiangzhining.
