Determination of Matrine in Matrine Ethosome Thermosensitive Gels by HPLC

VernacularTitle:高效液相色谱法测定苦参碱醇质体温敏凝胶中苦参碱含量
Author: Zhengde HUANG ; Ruwen LIN ; Xiaoping LIU
Publication Type:Journal Article
Keywords: Matrine ethosome thermosensitive gels; Chromatography,high performance liquid; Determination
From: Herald of Medicine 2016;35(5):521-523
CountryChina
Language:Chinese
Abstract: Objective To establish an HPLC method for determination of matrine in matrine ethosome thermosensitive gels. Methods Matrine was detected in matrine ethosome thermosensitive gels by using a YMC-Pack NH2 column(4.6 mm× 150 mm,5 μm),with the mobile phase containing acetonitrile-ethanol(9: 1).The flow rate was 0.8 mL·min-1 ,the column temperature( 25 ± 2) ℃ and the UV detection wavelength 215 nm. Results The peak area correlated with matrine concentration within the range of 1.00-100.00 μg·mL-1 linearly,and the average correlation coefficient was 0.999 2.The average recoveries rate of low,medium and high concentration of matrine were 98.16%,98.15%,98.00%,respectively,and the RSD were 0.30%,0. 69%,0. 71%,respectively. Conclusion The method is simple,sensitive and accurate. With the advantages of specificity,high precision,good stability and repeatability,the HPLC method can be used for the determination of matrine in matrine ethosome thermosensitive gels.