Determination of the antibacterial agent content of compound dexamethasone acetate cream and its antibacterial effect evaluation
10.3969/j.issn.1005-1678.2016.04.59
- VernacularTitle:复方醋酸地塞米松乳膏抑菌剂含量测定及其抑菌效力评价
- Author:
Lin LIN
- Publication Type:Journal Article
- Keywords:
compound dexamethasone acetate cream;
ethyl hydroxybenzoate;
content determination;
antimicrobial effectiveness
- From:
Chinese Journal of Biochemical Pharmaceutics
2016;36(4):186-188
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method to determine the antiseptics in compound dexamethasone acetate cream and evaluate the usage rationality of antimicrobial preservatives. Methods The content of ethyl hydroxybenzoate was calculated by external standard with HPLC.The effectiveness of antimicrobial preservatives was measured according to the preservative effectiveness test in ChP(2015edition) by inoculating 5 kinds of test microorganisms including Pseudomonas aeruginosa,Escherichia coli,Staphylococcusaureus,Candida albicans and Aspergillusniger.Results The linear ranges of ethyl hydroxybenzoate was 6.63-132.6 μg/mL ( r =0.999 7 ) .The average recoveries was 99.2%.The samples from different batche had different antimicrobial effectiveness due to the content of ethyl hydroxybenzoate.Conclusion The described method can be used in the determination of the preservatives of ethyl hydroxybenzoate in compound dexamethasone acetate cream.At present the use of antibacterial agent is arbitrary, it is necessary to strengthen research and supervision.
