Determination of naphthopyrone glycosides and anthraquinone aglycone in Cassia tora L by quantitative analysis of multi-components by single marker
10.3969/j.issn.1005-1678.2016.04.60
- VernacularTitle:一测多评法测定决明子中蒽醌苷元类和萘并吡喃酮苷类有效成分含量
- Author:
Xiqin WEI
;
Shijie WEI
;
Yanni MA
- Publication Type:Journal Article
- Keywords:
quantitative qnalysis of multi-components by single marker;
Cassia tora L;
naphthopyrone;
anthraquinone;
rubrofusarin-gentiobioside;
aurantio-obtusin;
chrysophanol;
physcion
- From:
Chinese Journal of Biochemical Pharmaceutics
2016;36(4):189-192
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a method of quantitative analysis of multi-components by single marker(QAMS) for determination of effective components of naphthopyrone glycosides and anthraquinone aglycone in Cassia tora L.Methods The separation was performed on a Sunfire C18 column (4.6 mm ×250 mm,5 μm), and column temperature 30 ℃.Aetonitrile-0.1% H3PO4 was used as the mobile phase with gradient elution.The detection wavelength was at 278 nm and 284 nm.Relative correction factor by determination of Between aurantio-obtusin and rubrofusarin-gentiobioside or chrysophanol or physcion.the method was evaluated for reproducibility, and the difference between calculated and measured values was compared. Results Rubrofusarin-gentiobioside, aurantio-obtusin, chrysophanol and physcion respectively 0.0503-1.5078, 0.3197-9.5899, 0.5070-15.2108, 0.4027-12.0814μg showed a good linear relationship, and the regression equation is Y =4.95X +2.01(R2 =0.9998),Y =1.03X +0.03(R2 =0.9999),Y=3.98X-0.12(R2 =0.9993),Y =4.81X +0.26(R2 =0.9996).The quantitative results of six batches of Cassia tora L by QAMS was basically consistent with that by external standard method.Conclusion The QAMS method was reliable and accurate, which might be used for the quality control of Cassia tora L.
