Dynamic analysis of the preparation of Huangqi injection

VernacularTitle:黄芪注射液制备的动态分析
Author: Yuedong ZHAO ; Danyang CHEN ; Qing WANG ; Wenjie HAN ; Qi ZHANG ; Haimin LEI ; Qiang LI
Publication Type:Journal Article
Keywords: Huang qi zhu she ye; Chromatography,high pressure liquid; Dynamic analysis
From: International Journal of Traditional Chinese Medicine 2016;38(5):433-435
CountryChina
Language:Chinese
Abstract: Objective To dynamically the preparation process of Huangqi injection and to verify the rationality and existing problems of the process. Methods The preparation was made by the current standard (WS3-B-3335-98) issued by Ministry of Health, and the solid amount of the key processes were measured. The HPLC separation was performed on a Agilent Zorbax Bio-C18 reversed-phase column (250 mm × 4.6 mm, 5μm) in gradient mode of acetonitrile-water with UV detection at 254 nm. The column temperature was kept at 25 ℃, and the flow rate of mobile phase was 1.0 ml/min. Results Solid amounts of different operations of Huangqi injection were measured accurately. The content of the third water extracts was only 6.1%, and the changes of HPLC pointed the content of the 12 peaks of the second peaks decreased obviously. Conclusion The technological rationality of Huangqi injection need to be verified and optimized, the dynamic analysis of HPLC describes the changes of chemical constituents of Huangqi injection qualitatively, which also provides a reference value for establishing its fingerprint.