HCMV-encoded miRNA during latent infection

VernacularTitle:人巨细胞病毒潜伏感染状态下编码miRNA的研究
Author: Xin SONG ; Yanyu ZHANG ; Jiang DENG ; Jun XIAO ; Bo GAO ; Ping MA ; Jingyuan BI ; Xipeng ZHOU ; Jinbo XU
Publication Type:Journal Article
Keywords: human cytomegalovirus; latent infection; deep-sequencing; bioinformatics; microRNA
From: Military Medical Sciences 2016;40(4):311-315
CountryChina
Language:Chinese
Abstract: Objective To explore humam cytomegalovirus(HCMV) encoded microRNAs during latent infection in order to help study HCMV virology and latent infection mechanisms.Methods A model of HCMV latent infection via THP-1 cells infected with HCMV was constructed.Deep-sequencing was performed using high-resolution Solexa sequencing platform.The secondary structure of the newly sequenced miRNA was predicted by RNAFold bioinformatics software. Results HCMV encoded various miRNAs during latent infection, including miR-US25-2-3p, miR-US25-2-5p, miR-UL112, miR-US25-1, miR-UL22A and PC-5p-148467 with a predicted length of 25 bp, named hcmv-miR-US33as-5p.Conclusion HCMV can express many types of miRNAs during latent infection.