Evaluation of immune protection by inactivated whole bacteria vaccine of Stenotro-phomonas maltophilia
10.7644/j.issn.1674-9960.2016.04.012
- VernacularTitle:嗜麦芽窄食单胞菌全菌灭活苗在小鼠体内的免疫保护作用
- Author:
Yao QIAN
;
Xueyi SHANG
;
Xueping TANG
;
Guangyang XU
;
Yongqiang JIANG
;
Yan LI
- Publication Type:Journal Article
- Keywords:
Stenotrophomonas maltophilia;
whole bacteria vaccine;
immune protection
- From:
Military Medical Sciences
2016;40(4):316-318
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the immunoprotection by the inactivated whole bacteria(IWB) of Stenotrophomonas maltophilia K279a in mice.Methods Mice were immunized by inactivated whole bacteria of S.maltophilia K279a made from formaldehyde.When the indicated the antibody titer of the mice reached the require level, the protective effect of the IWB was evaluated by performing the opsonophagocytic killing test in vitro and the poison attack experiments in vivo. Results It was found that IgG in serum of the immunized mice measured by ELISA was significantly increased after the second immune enhancement, and antiserum in vitro had strong phagocytic effect.Meanwhile, immunoprotection of the immunized groups was also significantly increased when challenged by S.maltophilia K279a.Conclusion Effective humoral immune response can be predominantly induced by the inactivated whole bacteria of S.maltophilia K279a, providing protection against challenge by S.maltophilia K279a in BALB/c mice.
