Simultaneous determination of metolazone and valsartan in beagle dog plasma using liquid chromatography-tandem mass spectrometry
10.7644/j.issn.1674-9960.2016.04.020
- VernacularTitle:LC－MS／MS同时测定比格犬血浆中美托拉宗和缬沙坦
- Author:
Meiling CHEN
;
Jiezhao ZHOU
;
Xiaohui CHENG
;
Zhiping LI
;
Rutao WANG
;
Wanzhi ZHANG
;
Danyu MEI
;
Xiuhua WANG
;
Ying LI
;
Xingguo MEI
- Publication Type:Journal Article
- Keywords:
LC-MS/MS;
metolazone;
valsartan;
beagle dog;
plasma
- From:
Military Medical Sciences
2016;40(4):346-349
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an LC-MS/MS method for simultaneous determination of metolazone and valsartan in beagle dog plasma.Methods The chromatographic separation was achieved on an Agilent Poroshell 120(2.1 mm ×30 mm × 2.7 μm)analytical column.The multiple reaction monitoring mode operating in positive ion was adopted in MS detection, and precursors to the product ion transitions of m/z 366.2/259, 436.2/291 and 423.4/207 were used to measure metola-zone, valsartan and internal standard ( losartan potassium) .Results The method was linear over the concentration range of 0.5 ng/ml-100 ng/ml for metolazone and 5-5000 ng/ml for valsartan, with the correlation coefficients ( r2 ) of 0.9937 and 0.9939, respectively.The average intra-day precision values ( RSD) were 2.09% -8.85% for metolazone and 2.36%-13.12%for valsartan.The matrix effect values were 87.73%-98.62%for metolazone and 99.03%-137.35%for valsartan.The average recovery was 75.74%-81.82%for metolazone and 83.89%-95.64%for valsartan.Conclu-sion This analytical method for metolazone and valsartan is simple, accurate and sensitive, so it can be used for pharma-cokinetic research of metolazone and valsartan immediate release tablets in beagle dogs.
