Effects of safflower yellow on angiotensin receptor II1 of kidney cells in diabetic nephropathy rats
10.3969/j.issn.2095-4344.2016.18.014
- VernacularTitle:红花黄色素对糖尿病肾病模型大鼠肾脏细胞血管紧张素Ⅱ1型受体的影响
- Author:
Yan GAO
;
Luliang YUAN
;
Haisong ZHANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(18):2677-2683
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Safflower yel ow is known to treat diabetic nephropathy, can protect kidney function, reduce lesions, delay or prevent the development of diabetic nephropathy.
OBJECTIVE: To further verify the effects of safflower yel ow on the kidney in rats with diabetic nephropathy and the impact of safflower yel ow on the expression of renal cel receptor of angiotensin II1.
METHODS: Thirty rats were equally and randomly divided into three groups: normal control group, model group and experimental group. In the experimental group and model group, rat models of diabetic nephropathy were established. At 1 week after model establishment, rats were daily intraperitoneally given safflower yellow injection 27.8 mg/kg, once a day. In the model and control groups, rats were daily intraperitoneally given an equal volume of saline. All rats were administered for 23 weeks. The kidney-related biochemical indicators and pathological changes in kidney tissue and the expression of angiotensin II1 type receptor on kidney tissue of rats were observed.
RESULTS AND CONCLUSION: (1) Hypertrophy index of experimental group and model group was significantly higher than that of control group (P < 0.05). (2) 24-hour proteinuria, glycosylated hemoglobin, total cholesterol, triglycerides, creatinine and urea nitrogen of model group were higher than that of control group (P < 0.05). 24-hour proteinuria, glycated hemoglobin, total cholesterol and urea nitrogen in experimental group were higher than that of control group (P < 0.05). 24-hour proteinuria, total cholesterol, triglycerides, creatinine and urea nitrogen in the experimental group were lower than that of model group (P < 0.05). (3) Glomerular hypertrophy, thickening of capillary basement membrane, cell proliferation, and mesangial widening were detected in the model group. Abnormal changes in the kidney structure were found in the control group. The extent of damage of kidney of histopathology in the experimental group was between the control group and the model group. (4) The expression level of angiotensin receptor II1 of experimental group and model group was higher than that of control group (P < 0.05). The expression level of angiotensin receptor II1 of experimental group was lower than that of model group (P < 0.05). These results indicated that safflower yellow has a protective effect on diabetic nephropathy; the mechanism of action may be associated with blocking kidney partial renin-angiotensin system.
