Vitreous cavity injection with erythropoietin protects optic nerve in a rat model of diabetic retinopathy
10.3969/j.issn.2095-4344.2016.18.016
- VernacularTitle:玻璃体腔注射促红细胞生成素保护糖尿病视网膜病变模型大鼠的视神经作用
- Author:
Yonghong ZHANG
;
Xiaomei ZHANG
;
Xiaoyi WEI
;
Liuping TANG
;
Daihua LIU
;
Yu LING
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(18):2690-2696
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Diabetes can lead to many complications. Of them, retinopathy is a common type. To explore the effect of erythropoietin in diabetic retinopathy, it is necessary to establish an animal model of similar pathologic features and high reproducibility in clinical retinal neovascularization.
OBJECTIVE: To investigate the protective effect of vitreous cavity injection of erythropoietin on optic nerve of rats with diabetic retinopathy.
METHODS: Rat models of diabetic retinopathy were established and injected with erythropoietin through vitreous space for 14 consecutive days. Results were compared between the model and normal control groups.
RESULTS AND CONCLUSION: (1) Transmission electron microscope: compared with the model group, retinal ganglion cel nucleus presented uniform red; cel membrane was intact; endoplasmic reticulum exhibited slight swel ing. A smal number of vacuoles were found in mitochondria around the nuclei. (2) Western blot assay and RT-PCR: compared with the model group, Caspase-3 and Bax protein expression was lower in the erythropoietin group (P < 0.05). Caspase-3 protein and Bcl-2 protein expression was significantly higher in the erythropoietin group than in the model group (P < 0.05). Significant differences in nuclear factor κB and Survivn gene expression were detected between the model and erythropoietin groups (P < 0.05). (3) Results verified that vitreous cavity injection of erythropoietin had neuroprotective effect on rats with diabetic retinopathy, which may be associated with the inhibitory effect on apoptosis.
