Effect of Probucol on Enzyme and Receptors of High Density Lipoprotein During Reverse Cholesterol Transportation in Experimental Rabbits With Atherosclerosis
10.3969/j.issn.1000-3614.2016.04.019
- VernacularTitle:普罗布考对动脉粥样硬化兔的高密度脂蛋白逆转运功能中酶蛋白和受体的影响
- Author:
Jiankai ZHONG
;
Yanxian WU
;
Yingwen CHEN
;
Chen LI
;
Wensheng LI
;
Zhigang GUO
- Publication Type:Journal Article
- Keywords:
Atherosclerosis;
Probucol;
Lipoproteins;
Lipoproteins,High density lipoprotein
- From:
Chinese Circulation Journal
2016;31(4):393-397
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effect of probucol on enzyme and receptors of high density lipoprotein (HDL) during reverse cholesterol transportation in experimental rabbits with atherosclerosis. Methods: A total of 24 New Zealand rabbits were randomly divided into 3 groups: Control group, the rabbits were fed by normal diet, High cholesterol group, the rabbits were fed by high cholesterol diet and Probucol group, the rabbits were fed by high cholesterol diet with probucol.n=8 in each group, all animals were treated for 12 weeks. Blood levels of lipids were examined by colorimetric method, serum lecithin cholesterol acyltransferase (LCAT) and cholesterol ester transfer protein (CETP) were detected by ELISA, expressions of ATP binding cassette transporter A1 (ABCA1) and scavenger receptor class B type I (SR-BI) in aortic plaque were measured by immunohistochemistry; the above indexes were compared among different groups.
Results: ① for blood lipids by mmol/L, the following indexes in Probucol group were lower than High cholesterol group: TC (15.95±1.51 vs 21.95±3.71), LDL-C (13.01±2.28 vs 17.90±3.51), HDL-C (0.56±0.10 vs 1.13±0.12), all P<0.01.② the following perimeters in High cholesterol group were lower than Control group: for CETP by μg/ml (1.24±0.54 vs 2.07±0.64), for LCAT by μg/ml (15.02±3.81 vs 27.01±8.26), all P<0.05; compared with High cholesterol group, Probucol group had increased CETP (3.43±1.01) and LCAT (38.10±7.96), all P<0.05. ③ positive expressions of ABCA1 and SR-BI in aortic plaque in Probucol group were higher than High cholesterol group: for ABCA1 by % (46.81±10.01 vs 24.10±8.48), for SR-BI by % (48.04±10.90 vs 18.61±6.77), all P<0.01.
Conclusion: Probucol may increase blood levels of LCAT, CETP via up-regulating the expressions of ABCA1, SR-B1 and elevating the reverse cholesterol transportation of HDL, therefore improve HDL function in experimental rabbits with atherosclerosis.
