Effects of Astragalus Polysaccharides on Damage of Keratinocytes in UVB Radiation Skin
10.3969/j.issn.1005-5304.2016.05.012
- VernacularTitle:黄芪多糖对中波紫外线辐射皮肤角质形成细胞损伤的影响
- Author:
Yang LI
;
Fangyu AN
;
Haixia MING
;
Yongqi LIU
- Publication Type:Journal Article
- Keywords:
Astragalus polysaccharides;
ultraviolet B;
HaCaT cells;
protective effect
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(5):44-46
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Astragalus polysaccharide (APS) on damage of keratinocytes in ultraviolet B (UVB) radiation skin in HaCaT cells; To preliminarily explore its mechanism of action.Methods HaCaT cells were treated by UVB irradiated for modeling. Blank control group, model group, UVB irradiation group and low-, medium- and high-dose APS interventional groups were set up. Each medication group was given relevant medicine. MTT assay was used to determine the cell activity; GSH-Px, CAT, SOD activity and MDA content were detected by biochemical colorimetric method; contents of TNF-α and IL-6 were detected by ELISA.Results Compared with the control group, the contents of SOD, CAT and GSH-Px in the model group decreased significantly, while the contents of MDA, TNF-α and IL-6 increased significantly (P<0.05); compared with the mode group, the contents of SOD, CAT and GSH-Px in the medication groups increased significantly, while the contents of MDA, TNF-α and IL-6 were reduced significantly (P<0.01).Conclusion APS can reduce the oxidative stress injury of UVB to HaCaT cells to some extent.
