Quantitative Analysis of Amygdalin inKuxingren Formula Granules and Study on Characteristic Chromatogram
10.3969/j.issn.1005-5304.2016.05.022
- VernacularTitle:苦杏仁配方颗粒中苦杏仁苷的定量分析及特征图谱研究
- Author:
Yingchun WANG
;
Jingmei MA
;
Si LI
;
Junshan LI
;
Zhenjiang LI
;
Liying NIU
- Publication Type:Journal Article
- Keywords:
KuxingrenFormula Granules;
HPLC;
amygdalin;
content determination;
characteristic chromatogram
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(5):87-90
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo establish an HPLC analytic method for amygdalin inKuxingrenFormula Granules and HPLC characteristic chromatogram.MethodsRP-HPLC method was adopted. The determination was performed on Dionex C18 column (4.6 mm×250 mm, 5μm) with acetonitrile-0.1% phosphoric acid solution (8:92) at the flow rate of 0.6 mL/min. The detection wavelength was set at 207 nm and sample size was 10μL. The column temperature was 30℃. The peak of amygdalin was set as refernce, and 10 batches of samples were analyzed. TCM Chromatogram Fingerprint Similarity Evaluation System (2004 A) was adopted to evaluate similatity.Results The linear equation of amygdalin wasY=6.176×10-7X?3.058×10-3, with a good linear relationship in the range of 0.122 5–1.225μg (r= 0.999 8). The average recovery rate was 98.75% (RSD=1.30%). There were 6 common peaks in the characteristic chromatogram ofKuxingrenFormula Granules, and the similarity of 10 batches of samples was higher than 0.981. ConclusionThe HPLC method is simple, accurate and reproducible, which can be used for the quality control of Kuxingren Granules.
