Preparation of a rat model of ulcerative colitis with spleen and kidney Yang deficiency
10.3969/j.issn.1005-4847.2016.02.002
- VernacularTitle:脾肾阳虚型溃疡性结肠炎大鼠模型的建立
- Author:
Yuhong WU
;
Yaqing XU
;
Hailong LI
;
Xiaoli CHENG
;
Haixia MING
;
Lei ZHANG
;
Fenglin LIU
;
Ruiqun WANG
- Publication Type:Journal Article
- Keywords:
Ulcerative colitis;
Spleen and kidney Yang deficiency;
Enzyme linked immunosorbent assay;
Rats
- From:
Acta Laboratorium Animalis Scientia Sinica
2016;24(2):116-119
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rat model of ulcerative colitis ( UC) with spleen and kidney Yang deficien-cy.Methods The rat model of ulcerative colitis with spleen and kidney Yang deficiency was established by oral adminis-tration of Rhubarb decoction, intramuscular injection of hydrocortisone, and combined with ethanol enema of TNBS (2,4, 6-trinitrobenzene sulfonic acid).Sixty Wistar rats ( body weight 180 ±20 g, male:female=1∶1) were randomly divided into blank control group and groups of UC models with spleen kidney Yang deficiency for 7 days, 14 days and 21days.The serum levels of FT3, FT4, and T of the rats were detected by ELISA assay.Results Compared with the blank control group, the serum levels of FT3, FT4, and T in the groups of UC rat models with spleen kidney Yang deficiency for 7 days, 14 days and 21days were decreased to a different extent (P<0.05), especially, decreased dramatically in the model group for 21 days.Conclusions FT3, FT4, and T are sensitive indexes with spleen and kidney Yang deficiency.The detection of serum levels of FT3, FT4, and T can better verify the spleen and kidney Yang deficiency in the rats, and prove that the spleen and kidney Yang deficiency type UC animal model is successfully prepared.
