Laboratory proficiency evaluation of the detection ability of malic enzyme 1 and isocitrate dehydrogenase 1 in mouse kidneys
10.3969/j.issn.1005-4847.2016.02.017
- VernacularTitle:实验小鼠肾匀浆中苹果酸酶-1和异柠檬酸脱氢酶-1实验室检测能力验证结果评价
- Author:
Hong WANG
;
Jie WEI
;
Pengli YU
;
Wei GONG
;
Bingfei YUE
- Publication Type:Journal Article
- Keywords:
Capacity evaluation;
Laboratory animal;
Malic enzyme 1;
Isocitrate dehydrogenase 1
- From:
Acta Laboratorium Animalis Scientia Sinica
2016;24(2):199-203
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the detection capacity of malic enzyme 1 and isocitrate dehydrogenase 1 ( Mod1&Idh1) in the laboratory animal monitoring laboratories in China in order to understand the detection capacity of la-boratories and to improve the detection level of laboratory animals’ quality.Methods Based on the program approved by CNAS, samples preparing, homogeneity test and stability test of malic enzyme 1 and isocitrate dehydrogenase 1 in the mouse kidneys were carried out.Standard operation procedure and samples with random numbers were distributed to the la-boratories.The laboratories should submit the result reports before the time limit expires.If the laboratory reports were the same with the standard results, the laboratories will receive satisfactory remark.If laboratory reports were not the same with the standard results, the laboratories will receive unsatisfactory remark.If a laboratory did not submit report, the laboratory will also receive unsatisfactory result.Results Eight laboratories out of 10 (80%) enrolled laboratories reported satisfac-tory experiment results, and two laboratories (20%) presented unsatisfactory results.Conclusions The whole detection level of laboratories in Mod1 &Idh1 is relatively high in the laboratory animals monitoring laboratories in China.It can re-flect the detection level of laboratories to conduct the laboratory capacity evaluation.
