Content Determination of Protodioscin and Diosgenin in Dioscoreae Hypoglaucae Rhizoma from Different Habitats by HPLC
10.3969/j.issn.1005-5304.2016.05.026
- VernacularTitle:HPLC测定不同产地粉萆薢中原薯蓣皂苷和薯蓣皂苷含量
- Author:
Yanyan SHAO
;
Xiao XIAO
;
Lingfang WU
;
Dan ZHU
;
Guanghui YANG
;
Xiaoxue ZHANG
;
Lanzhen ZHANG
- Publication Type:Journal Article
- Keywords:
Dioscoreae Hypoglaucae Rhizoma;
place of production (TCD);
protodioscin;
diosgenin;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(5):104-106
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method for the content determination of protodioscin and diosgenin in Dioscoreae Hypoglaucae Rhizoma from different habitats.Methods The chromatographic separation was performed on an Agilent C18 column (4.6 mm×150 mm, 5μm) with mobile phase of acetonitrile-water solution with gradient elution at the flow rate of 0.8 mL/min; the detection wavelength was 208 nm; the column temperature was 30℃; the injection volume was 20μL.Results Protodioscin showed a good linear relationship among the range of 1.73–8.64 μg (r=0.999 6), with the average recovery of 101.98% (RSD=1.53%); Diosgenin showed a good linear relationship among the range of 1.03–8.20μg (r=0.999 1), with the average recovery of 101.60% (RSD=2.41%). The contents of protodioscin and diosgenin in Dioscoreae Hypoglaucae Rhizoma from 10 different habitats were in the range of 0.89%–2.24% and 0.75%–3.22%, respectively.Conclusion The method is simple, accurate and with repeatability, which can be used as quality control method of Dioscoreae Hypoglaucae Rhizoma.
