Seminiferous capsule extract effect on the proliferation of spermatogonial stem cells
10.3969/j.issn.2095-4344.2016.14.019
- VernacularTitle:养精胶囊提取液对精原干细胞增殖的影响
- Author:
Juntao LI
;
Peihai ZHANG
;
Xiaowei QU
;
Zhengsheng LI
;
Songwei LI
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(14):2104-2109
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Chinese herbs for kidney nourishment can promote the proliferation and differentiation of spermatogonial stem cel s. OBJECTIVE:To verify the effect of seminiferous capsule extract on spermatogonial stem cel proliferation. METHODS:Spermatogonial stem cel s were isolated from the testis of male mice and synchronized by serum-free medium fol owed by an addition of 10, 50, 100 mg/L seminiferous capsule extracts. After 24 hours of culture, viability, proliferation and cel cycle of spermatogonial stem cel s were observed. RESULTS AND CONCLUSION:Compared with the control group, seminiferous capsule extracts promoted the cel number, viability and proportion at S stage. The number of BrdU-labeled spermatogonial stem cel s was increased significantly after intervention with seminiferous capsule extracts, especial y at the concentration of 50 mg/L. These findings indicate that seminiferous capsule extracts can promote the proliferation and viability of spermatogonial stem cel s.
