Rubella virus genotype 2B epidemic strains circulating in Beijing, 2015
10.3760/cma.j.issn.0254-5101.2016.03.002
- VernacularTitle:北京市2015年风疹病毒发现2B基因型流行株
- Author:
Xiali YU
;
Meng CHEN
- Publication Type:Journal Article
- Keywords:
Rubella virus;
Genotype;
Phylogenetic analysis
- From:
Chinese Journal of Microbiology and Immunology
2016;36(3):166-170
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the genotypes and the variation trend of rubella virus strains circulating in Beijing and to provide a scientific guidance for the prevention and control of rubella.Methods The viral nucleic acids were extracted from diagnostic pharyngeal swab specimens collected by Beijing rubella laboratory.The 739 nucleotides of rubella E1 gene were amplified by reverse transcription-polymerase chain reaction ( RT-PCR) .The amplified products were sequenced and analyzed.Genotypes of the rubella virus strains circulating in Beijing and variations in their nucleotides and amino acids were analyzed by comparing with those of the WHO recommended rubella virus reference strains.Results The target nucleotide se-quences of 10 rubella virus strains were obtained for molecular epidemiological study.All of the 10 strains belonged to 2B genotype and formed an independent branch according to the phylogenetic tree based on the 739 nucleotide fragments of E1 gene.Most of the nucleotide mutations occurred in the 10 strains were non-sense mutations.The amino acid sequences of the circulating strains were highly conservative.No significant mutations in the antigenic sites were identified in the rubella virus strains circulating in Beijing except that one amino acid mutation from Ser to Phe in the No.282 amino acid of E1 protein was identified in the hemag-glutination inhibition and neutralizing site of one strain.Conclusion This study indicated that the rubella virus genotype 2B epidemic strains were isolated in Beijing.
