Efficacy of using Xinjiang wild Artemisia rupestris L.crude polysaccharides as an immunologic adju-vant for influenza virus vaccine
10.3760/cma.j.issn.0254-5101.2016.03.013
- VernacularTitle:新疆野生一枝蒿粗多糖作为流感病毒疫苗佐剂的免疫效果
- Author:
Danyang WANG
;
Yu YANG
;
Bing ZHAO
;
Feng GAO
;
Xucheng FAN
;
Ailian ZHANG
- Publication Type:Journal Article
- Keywords:
Influenza virus vaccine;
Artemisia rupestris L.;
Polysaccharide;
Adjuvant
- From:
Chinese Journal of Microbiology and Immunology
2016;36(3):230-234
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the efficacy of using Xinjiang wild Artemisia rupestris L. crude polysaccharides ( WARCP) as an immunologic adjuvant for influenza virus vaccine( IVV) .Methods ICR mice were subcutaneously immunized with 0.3 μg of IVV and 1.5 μg of IVV alone or co-administered with 200 μg of WARCP on 0 d and 14 d.Antibody levels in serum samples were detected by using indirect ELISA.MTT method was used to measure the proliferation of splenocytes.The growth conditions of mice were observed as well.Results No significant differences in the body weight were observed between mice from different groups (P>0.05).The levels of influenza virus-specific IgG, IgG1 and IgG2a were signifi-cantly increased in mice injected with WARCP adjuvant (P<0.05).The levels of IgG antibody in mice im-munized with low-dose of IVV and WARCP were significantly higher than those in mice immunized with high-dose of IVV alone (P<0.05), indicating at least 80% reduction in vaccine dosage by adding WARCP as adjuvant.Moreover, WARCP significantly promoted the proliferation of lymphocytes (P<0.05).Conclu-sion Adding WARCP to IVV enhanced the efficacy of IVV by boosting humoral and cellular immunity re-sponses with the advantages of high safety and dose-sparing.This study suggested the possibility of using WARCP as a novel immunologic adjuvant for influenza virus vaccine.
