Expressions of glial fibrillary acidic protein in the prefrontal cortex, hippocampus, and amygdala in post-stroke depression in rats
10.3760/cma.j.issn.1673-4165.2016.01.010
- VernacularTitle:卒中后抑郁大鼠前额叶皮质、海马和杏仁核胶质纤维酸性蛋白表达
- Author:
Yun LI
;
Wei WANG
;
Shu LI
;
Yangchao LI
;
Ning YANG
- Publication Type:Journal Article
- Keywords:
Stroke;
Brain Ischemia;
Depression;
Glial Fibrilary Acidic Protein;
Prefrontal Cortex;
Hippocampus;
Amygdala;
Disease Models,Animal;
Rats
- From:
International Journal of Cerebrovascular Diseases
2016;24(1):58-62
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expressions of glial fibrilary acidic protein (GFAP) in the prefrontal cortex, hippocampus and amygdala in post-stroke depression (PSD) in rats. Methods Healthy adult SD rats w ere randomly divided into a normal group, a depression group, a stroke group, and a PSD group ( n=5 in each group). A model of focal cerebral ischemia w as induced by the intraluminal suture method in the stroke group;a rat chronic stress depression model was induced by using chronic unpredicted mild stress (CUMS) combined w ith single housing in the depression group;a model of focal cerebral ischemia w as induced by the intraluminal suture method in the PSD group. A rat PSD model w as induced by CUMS and single housing at 1 week after operation. The sucrose preference test (SPT) was performed in each group at day 1, 8, 15, and 29 after the first CUMS, and the open-field test ( OFT ) w as used to evaluate the depressive behaviors. Immunofluorescence staining was used to detect the expression of GFAP in the prefrontal cortex, and hippocampus and amygdala at day 29. Results At day 29 after CUMS, the sucrose solution consumption in SPT and the scores of horizontaland vertical movement in OFT in the depression group and PSD group w ere decreased significantly compared w ith the normal group and the stroke group (al P<0.05);the numbers of GFAP immunopositive cel s in the prefrontal cortex, hippocampus, and amygdala in the PSD group w ere significantly less than those in the normal group, depression group and stroke group (al P<0.05), and there w ere no significant differences among the normal group, depression group and stroke group (al P>0.05). Conclusion The decreased expression of GFAP in the prefrontal cortex, hippocampus, and amygdale may play a certain role in the process of PSD.
