Assessment of pain in rats with chronic pancreatitis using behaviour and the rat grimace scale
- VernacularTitle:面部表情疼痛量表和行为学评估慢性胰腺炎大鼠的疼痛
- Author:
Zhiyun LIU
;
Lin SHEN
;
Haibo QIU
;
Qianbo CHEN
;
Jinchao SONG
;
Zhijie LU
- Publication Type:Journal Article
- Keywords:
Chronic pancreatitis;
Abdominal hypersensitivity;
Rat grimace scale
- From:
The Journal of Clinical Anesthesiology
2016;32(4):380-382
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether rat grimace scale (RGS)could be used to assess pain in chronic pancreatitis,so as to provide evidence for pain research and clinical assessment of rat pain.Methods Twenty-eight adult male wister rats were evenly randomized into two groups (n =14):an experimental group and a control group.The experimental group was intravenously given 8 mg/kg body weight dibutyltin dichloride (DBTC)to induce chronic pancreatitis,and the control group was injected with ethanol and glycerin solution.Abdominal hypersensitivity,RGS scores and weight at different time points was detected.HE staining was used to detect the histological changes of pancre-atic tissue.Results Compared with the control group,the rats in the experimental group showed chro-nic inflammation in pancreatic tissue in two weeks.There was a significant increase in the number of abdominal withdrawals (P < 0.001 )and RGS in the experimental group.Conclusion Rat grimace scale might lead to a successful transition of basic science findings into clinical application.
