Bactericidal effect of a novel peptide on oral microorganisms
10.16571/j.cnki.1008-8199.2016.04.005
- VernacularTitle:新型优化多肽对口腔常见微生物的杀菌作用
- Author:
Xiu YING
;
Yingming SUN
;
Rui TAO
;
Mengjie ZHANG
;
Xiu JIANG
- Publication Type:Journal Article
- Keywords:
Antimicrobial peptide;
Streptococcus mutans;
Streptococcus sanguinis;
Caries
- From:
Journal of Medical Postgraduates
2016;29(4):359-363
- CountryChina
- Language:Chinese
-
Abstract:
Objective Antimicrobial peptides are the focus of recent research in oral microbiology .This study aimed to eval-uate the activity of a novel antimicrobial peptide pm 11 against oral microorganisms and its action mechanisms . Methods We ana-lyzed the effect of pm11 on oral microorganisms and determined its antimicrobial activity in the saliva environment by measuring its min -imal inhibitory concentration (MIC), minimal bactericide concentration (MBC), and bactericidal kinetics.We observed its bacteri-cidal activity on the biofilms of streptococcus mutans by confocal laser scanning microscopy (CLSM) and the structural changes in the bacterial membrane by scanning electron microscopy (SEM). Results The antimicrobial activity of pm11 varied greatly against dif-ferent oral microorganisms , with its MIC values ranging from 2 μg/mL to 256 μg/mL and its MBC values from 2 μg/mL to >256μg/mL.The bactericidal kinetics showed a decreasing survival rate of bacteria with the lengthening of the intervention time .The inhib-itory-zone diameters exhibited no significant indifference between the water solution and the sterile saliva solution .CLSM revealed an increased number of dead bacteria in the pm 11-treated biofilms , while SEM manifested obvious changes in the shape of the bacteria membrane treated with pm11. Conclusion Our findings suggest that pm11 has a broad spectrum of antimicrobial activities on oral mi-croorganisms and a potential value of clinical application .
