The changes of follicular helper T cells and their associated molecules in patients with malignant lymphoid diseases and their significances
10.3760/cma.j.issn.1009-9921.2016.03.007
- VernacularTitle:滤泡辅助性T细胞和相关分子在淋系恶性疾病中的变化及其意义
- Author:
Dongming ZHOU
;
Yanxia XU
;
Yuqing YUAN
;
Ping CHEN
;
Yu SUN
;
Jinxiang FU
- Publication Type:Journal Article
- Keywords:
Follicular helper T cells;
CXCR5;
Inducible costimulator;
Programmed death 1;
Interleukin 21
- From:
Journal of Leukemia & Lymphoma
2016;25(3):157-162
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of follicular helper T cells (Tfh cells) and Tfh cells associated molecules in the peripheral blood (PB) of patients with malignant lymphoid diseases (MLD) dynamically, and explore their roles on pathogenesis of the diseases. Methods Fifty-five patients with MLD were enrolled in this study,including 9 patients with acute lymphocyte leukemia (ALL), 30 patients with non-Hodgkin lymophoma (NHL) and 16 patients with multiple myeloma (MM), and 10 healthy controls (NC) of similar age were also enrolled. The percentage of CD4+CXCR5+cells (Tfh cells) and expression of ICOS+, PD1+among the T cells were detected by flow cytometry (FCM), while the levels of interleukin 21 (IL-21) in plasma were detected by ELISA tests. Results The percentage of Tfh cells and expression of ICOS and/or PD-1 in PB of all untreated patients were significantly higher than those of NC (all P< 0.01), and MM group< ALL group 0.05), and apparently lower than those who achieved PR (P< 0.05). The percentages of Tfh cells in patients with T-ALL and T-NHL were higher than those in patients with B-ALL and B-NHL (P> 0.05), and much higher than NC (P< 0.01). The concentration of IL-21 in patients were much higher than that in NC [(326.56±32.44) pg/ml] (P<0.01), and MM group
