The expression of protection of telomeres 1 in acute myeloid leukemia and its significance
10.3760/cma.j.issn.1009-9921.2016.03.009
- VernacularTitle:端粒保护蛋白1在急性髓系白血病中的表达及其意义
- Author:
Fang YE
;
Ruixia MA
;
Zhenhua QIAO
;
Yanhong TAN
;
Jie WANG
- Publication Type:Journal Article
- Keywords:
Leukemia,myeloid,acute;
Protection of telomere 1;
Real-time fluorescence quantitative polymerase chain reaction;
Blotting,Western
- From:
Journal of Leukemia & Lymphoma
2016;25(3):169-173
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the relationship between the expression of protection of telomeres 1 (POT1) and the pathogenesis of acute myeloid leukemia (AML). Methods 62 patients with de novo AML (case group) and 10 patients with iron deficiency anemia (control group) were enrolled in this study. The quantitative real-time polymerase chain reaction (PCR) and Western blot were used to detect the expression of POT1 in AML patients. Results There were 62 de novo AML patients, including 2 cases M1, 14 cases M2, 12 cases M3, 14 cases M4, 17 cases M5, 2 cases M6 and 1 case AML without classification. According to the risk stratification, high risk group (24 cases), medium risk group (22 cases) and low risk group (16 cases) were divided. Compared with that in the controls, POT1 expression levels in patients with AML were significantly decreased both in mRNA and protein level (P< 0.05). The relative expression levels of POT1 mRNA and protein in patients with M2, M4 and M5 were significantly lower than those in the controls (P< 0.05). The expression levels of POT1 in high risk group, medium risk group and low risk group were significantly decreased than those in the controls (P<0.05). Compared with that in the controls, The relative POT1 mRNA expression was significantly decreased in M3 patients (P< 0.05), but not in protein level. POT1 protein expression was showed both in the cytoplasm and nucleus. There was no significant difference of the expression of POT1 protein between cytoplasm and nucleus (P> 0.05). Conclusions POT1 may be involved in the pathogenesis of AML. POT1 protein expresses in both cytoplasm and nucleus, and the regulatory mechanism may be related to the telomere length.
